All raw sequencing data from the MBQC-baseline is available at SRA BioProject SRP047083. This file table additionally provides completely raw data as deposited by each of the MBQC-baseline’s sample handling labs. This includes Illumina 16S sequences as provided by each lab and (anonymized) manifests linking each file to one (if demultiplexed) or more originating biospecimens. Sample IDs and metadata are as provided above and in the MBQC supplement.
Between the MBQC-baseline’s sample handling / data generation phase and its bioinformatics phase, all sequence data were deposited by the handling labs and re-blinded prior to distribution for bioinformatics. This file table provides the raw, re-blinded sequence data as distributed to bioinformatics teams. Each handling lab is anonymized, and each sample is centrally demultiplexed and identified by the “Bioinformatics.ID” field as provided above and in the MBQC supplement.
This file table provides completely raw data as deposited by each of the MBQC-baseline's bioinformatics labs. This includes, at the least, an OTU table identified using Greengenes identifiers and a Newick-formatted phylogeny incorporating any additional de novo OTUs. Some labs chose to also deposit supplemental files such as processing scripts or protocol documentation.